Fig. 5

TRIM62 suppresses EMT by inhibiting c-Jun/Slug signaling in cervical cancer. a Representative IHC images of TRIM62 and EMT markers (α-Catenin and Vimentin). Original magnification: ×400. b Western blot analysis of α-Catenin and Vimentin expression in CC cells with or without TRIM62 overexpression. Beta-actin was used as a loading control. c Representative immunofluorescence images showed that TRIM62 affects the cellular morphology of CC cells. Cell nuclei were stained with DAPI (blue) and cytoskeleton (F-actin) were stained with rhodamine-phalloidin (red). Compared with the control cells respectively, SiHa-TRIM62 and HeLa-TRIM62 cells which overexpressed TRIM62 displayed cobblestone-like appearance with decreased F-actin fibers. Original magnification: ×400. d 10-Pathway Reporter Array showed the signaling changes in TRIM62-overexpressed cells compared with their corresponding controls. **, P < 0.01. e Protein expression levels of c-Jun and Slug detected by western blot were shown in indicated cells. f Protein levels of normal and phosphorylated form of JNK1 detected by western blot were shown in indicated cells