Fig. 6

Rescue of c-Jun abrogates inhibition of TRIM62 on cell migration and invasion. a Expression of c-Jun protein in SiHa-NC, SiHa-TRIM62, SiHa-TRIM62 + c-Jun, HeLa-NC, HeLa-TRIM62 and HeLa-TRIM62 + c-Jun cells was detected by western blot. b Western blot detected the expression level of α-Catenin and Vimentin in TRIM62-overexpressed cells after overexpression of c-Jun. c Representative immunofluorescence images showed that epithelial phenotype-like morphology of TRIM62-overexpressed cells changed back to mesenchymal phenotype-like morphology after rescuing the expression of c-Jun. Cell nuclei were stained with DAPI (blue) and cytoskeleton (F-actin) were stained with rhodamine-phalloidin (red). Original magnification: ×400. d The transwell migration and transwell invasion assays were performed to investigate the effect of c-Jun overexpression on the migration and invasion abilities of TRIM62-overexpressed CC cell lines. The inhibited migration and invasion by gain of TRIM62 were significantly abrogated after overexpression of c-Jun in TRIM62-overexpressed cells. Original magnification: ×100. *, P < 0.05; **, P < 0.01