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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Leptin promotes epithelial-mesenchymal transition of breast cancer via the upregulation of pyruvate kinase M2

Fig. 1

Leptin promoted epithelial-mesenchymal transition in breast cancer cell lines. a Western blotting analysis with specific antibodies demonstrated that Ob-Rb and Ob-Rt were expressed in MCF-7, SK-BR-3, and MDA-MB-468 cells. b An immunofluorescence assay showed leptin receptor expression on the membrane of breast cancer cells. Original magnification: ×400 c Morphological changes of breast cancer cells treated with leptin. The image showed cell-cell junction dissolution and loss of apical-basolateral cell polarity in MCF-7, SK-BR-3, and MDA-MB-468 cells after treatment with 200 ng/mL leptin. Original magnification: ×200 d Western blotting analysis demonstrated that leptin treatment upregulated vimentin and fibronectin expression, but downregulated E-cadherin expression in MCF-7, SK-BR-3, and MDA-MB-468 cells. All images were representative examples from three independent experiments

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