Fig. 2
Leptin increased PKM2 expression. (a–f) Serum-starved MCF-7 and SK-BR-3 cells were exposed to concentrations of leptin ranged from 50 ng/mL to 200 ng/mL or 200 ng/mL leptin for 0 h to 24 h. Q-PCR and Western blotting analysis demonstrated that leptin promoted PKM2 mRNA and protein expression in a dose- and time-dependent manner in MCF-7 and SK-BR-3 cells