Fig. 2

HBX-mediated expression of IL-7R in hepatoma cells. a RT-PCR (top) and western blot (down) used to measure the effect of HBX, HBS, preS1, preS2, HBC, HBe, and HBP on the expression of IL-7R in Huh-7 cells. b RT-PCR and western blot were used to measure the effect of different viral genes on the expression of IL-7R in HepG2 cells. c and d The expression of IL-7R gene in hepatoma cells was detected by RT-PCR and western blot after transfection with pUC-18 plasmid (Huh-7-Mock and HepG2-Mock), pUC-18-HBV1.2 plasmid (Huh-7-HBV and HepG2-HBV), and pUC-18-HBV1.2-HBX△ plasmid (Huh-7-HBX△ and HepG2-HBX△) for 48 h. e and f RT-PCR and western blot were used to examine the expression of IL-7R gene in hepatoma cells stably transfected with pcDNA3.1 plasmid (Huh-7-3.1 and HepG2-3.1) and pcDNA-HBX plasmid (Huh-7-HBX and HepG2-HBX). g Immunohistochemistry analysis was used to measure the expression of HBX and IL-7R in HBV-related hepatocellular carcinoma (HCC) tissues (n = 40) and adjacent tissues (n = 10), as well as correlation between HBX and IL-7R expression in HBV-related HCC tumors