Fig. 2

Gal-1 expression affects EMT in GC cells. a CM from NGFs (CM-NGFs), CAFs (CM-CAFs), Over cells (CM-Over) and Down cells (CM-Down) was harvested. MGC-803 cells were then treated with serum-free medium (st) or designated CM for 72 h, and the expression of E-cadherin, vimentin and Gli1 monitored by western blot and qRT-PCR. b Gal-1 promotes the proliferation of MGC-803 cells. c Invasion assay. GC cells treated with CM-Over exhibited significantly enhanced invasion abilities after 18 h in a scratch assay. Bars represent the mean number of invaded GC cells (six fields/sample). *P < 0.05. d MGC-803 cells following treatment for 48 h with designated CM (original magnification × 100). *P < 0.05 when compared with st