Fig. 1

miR-193a was downregulated in lung cancer cells due to DNA hypermethylation. a The expression of miR-193a was detected in several lung cancer cell lines and normal lung epithelial cell line BEAS-2B by qRT-PCR. b The expression of miR-193a was measured in lung cancer tissues and adjacent non-tumor tissues. n = 62. c The expression of miR-193a was detected in lung cancer tissues with metastasis (n = 24) or without metastasis (n = 38). d CpG islands around the region encoding pre-miR-193a were analyzed by MethPrimer software. e The methylation status of miR-193a was analyzed by MSP in several lung cancer cell lines and BEAS-2B. Ma: DNA marker; B: Blank; P: positive control of methylated DNA. Bands of ‘M’ or ‘U’ are PCR products from methylation-specific or unmethylation-specific primers, respectively. f–k The methylation status of miR-193a was analyzed by MSP in 27 lung cancer tissues (f–h) and 27 adjacent non-tumor tissues (i–k). l The expression of miR-193a was detected in A549 and H1299 cells treated with 5 μM AZA for 3 and 5 days. *P < 0.01 versus untreated cells