Fig. 6

Overexpression of miR-193a partly reverses TGF-β1-induced EMT. A549 and H1299 cells were treated with 10 ng/ml TGF-β1 for 3 days. a Immunofluorescence staining was performed for epithelial marker E-cadherin and the mesenchymal markers fibronectin and vimentin in A549 cells. b Morphologic changes associated with EMT at 3 days were shown in the phase contrast images. c Western blot for E-cadherin, fibronectin, and vimentin in A549 and H1299 cells treated with TGF-β1 for 3 days. d and e A549 and H1299 cells were transfected with LVX-NC or LVX-miR-193a, followed with treatment with TGF-β1 for 3 days. Immunofluorescence staining assay was performed for E-cadherin in these cells. f and g The expressions of miR-193a (f) and WT1 (g) were measured in A549 and H1299 cells treated with TGF-β1 for 3 days by qRT-PCR and western blot, respectively