Fig. 5
From: Efficacy and safety of aflibercept in in vitro and in vivo models of retinoblastoma
Aflibercept treatment suppressed retinoblastoma subretinal invasion and increased apoptosis in an orthotopic xenotransplantation model. a Representative H&E staining of the orthotopic xenotransplantation model. Subretinal invasion sizes were smaller in aflibercept-treated mice (n = 14) than in saline-injected group (n = 8). Original magnification, ×40; scale bar, 500 μm. b Quantification of subretinal invasion. Retinoblastoma subretinal invasion was significantly reduced after aflibercept treatment compared with the saline-injected group (* P < 0.05, ** P < 0.01). c Representative TUNEL staining (green) of the orthotopic xenotransplantation model. TUNEL positive cells were significantly increased in aflibercept treatment group. Original magnification, ×200; scale bar, 200 μm. d Quantification of TUNEL positive apoptosis cell number (* P < 0.05)