Fig. 4

Identification of ROS-associated genes by Human Oxidative Stress Plus PCR Array. a The gene screening of oxidative stress-related proteins. HepG2 cells were treated with B5G9 (6 μM) for 3 h, the RT2 Profiler PCR Array was performed (left panel). The fold change values of these genes were calculated (right panel). The changes of HMOX1 (b), SPINK1 (c), COX-2 (d) DUSP1 (e) and SOD2 (f) gene levels were consequences of ROS overload. HepG2 cells were treated with B5G9 (6 μM) in the presence or absence of NAC (20 mM) or MnTBAP (200 μM) for 3 h, the fold changes of these genes were detected by RT-PCR. ^* P ≤ 0.05, ^** P ≤ 0.01, ^*** P ≤ 0.001. g B5G9 had no effect on mRNA level of SOD1 and SOD3. HepG2 cells were treated with B5G9 (6 μM) in the presence or absence of NAC (20 mM) for 3 h, the fold changes of these genes were detected by RT-PCR