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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Patient-derived xenografts: a relevant preclinical model for drug development

Fig. 1

Figure show the take rate obtained by the implantation of tumor fragments from patients with colorectal cancer in different strains of immunodeficient mice. In particular, fragments from primary tumors of patient 128 (a) and of patient 130 (b) or from a metastatic limph node of patient 131 (c) were placed in medium supplemented with antibiotics, diced into 15–20 mm3 pieces, coated in Matrigel and implanted by a small incision and subcutaneous pocket made in one side of the lower back into different mice (F0). After tumor mass formation, at the indicated day, tumors were passaged (F1) and expanded in large cohorts for drug sensitivity experiments (F2). All animal procedures were approved by the ethics committee of the Regina Elena National Cancer Institute (CE/534/12) and were in compliance with the national and international directives (D.L. March 4, 2014, no. 26; directive 2010/63/EU of the European Parliament and of the council; Guide for the Care and Use of Laboratory Animals, United States National Research Council, 2011)

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