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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: GANT61, a GLI inhibitor, sensitizes glioma cells to the temozolomide treatment

Fig. 2

GANT61 inhibited the survival of human glioma cells in vitro. a Chemical structure and molecular weight of GANT61. b U87 and U251 glioma cells were treated with GANT61 (5 μM) for 48 h. Gli1 and Gli2 expression were determined by Western blot and (c) qRT-PCR analysis. GAPDH was used as the loading control. d U87 and U251 cells were cultured in 96-well plates, respectively. Relative proliferation of glioma cells treated with different doses of GANT61 for different durations were determined by CCK-8. GANT61 treatment significantly decreased cells proliferation in a dose- and time-dependent manner. e, f GANT61 inhibited invasion. Transwell assay showed that the invasive capability of U87 or U251 cells treated with GANT61 (5 μM) was weaker than the control in a dose-dependent manner. g, h GANT61 induced apoptosis. U87 and U251 cells were either solvent-treated (Control) or challenged with GANT61 for 36 h, and then, cell apoptosis was examined by flow cytometry assay using the Annexin V/PI. Annexin V analysis showed that glioma cells treated with GANT61 displayed greater apoptosis than the control group. Data are from one of three representative experiments. The error bars represent standard error. Data are shown as mean ± SEM for the three replicates. Statistical significance levels are indicated as *P < 0.05, **P < 0.01, ***P < 0.001 compared with control group

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