Fig. 5
From: GANT61, a GLI inhibitor, sensitizes glioma cells to the temozolomide treatment
GANT61 increased apoptosis of glioma cells treated with TMZ. Apoptosis of cells under different treatments were detected by flow cytometric analysis with Annexin V-FITC/PI staining. a U251 and (b) U87. Left: The representative quadrantal diagrams showing results of flow cytometric analysis. Right: Histogram manifested average apoptosis rates in cell groups of different treatments. GANT61 and TMZ treatment resulted in only a slight increase in apoptosis. *P < 0.05. Combined GANT61 and TMZ treatment resulted in a significant increase in early apoptosis rate compared to cells treated with GANT61 or TMZ alone, *P < 0.05, **P < 0.01. All data were representative of three independent experiments