Fig. 7
From: GANT61, a GLI inhibitor, sensitizes glioma cells to the temozolomide treatment
GANT61 enhanced the DNA damage effect initiated by TMZ treatment. a Western blot analysis showed an obviously decreased expression of Gli1 and Gli2 in cells treated with a combination of GANT61 and TMZ, compared to TMZ-treated U251 and U87 glioma cells. Cells were continuously exposed to 5 μM GANT61 or 100 μM TMZ for up to 48 h. GAPDH was used as the loading control. b U87 and U251 cells were treated with 5 μM GANT61, 100 μM TMZ or a combination of the two treatments for 48 h. The γH2AX levels were measured by immunoblot assay. c Representative comet staining images in U87 and U251 cells after different treatments (5 μM GANT61, 100 μM TMZ or both). d Quantitative assessment of comet tail moment. **P < 0.01