Fig. 8
From: GANT61, a GLI inhibitor, sensitizes glioma cells to the temozolomide treatment
GANT61 abrogated the MGMT, Notch1, and Hes1 overexpression induced by TMZ treatment. a qRT-PCR analysis of MGMT, Notch1, and Hes1 mRNA expression after incubation with GANT61, TMZ, GANT61 + TMZ, Shh, and Shh + TMZ for 48 h. Under TMZ, Shh or Shh + TMZ treatment, MGMT, Notch1, and Hes1 mRNA levels were much higher than the indicated treatment (TMZ vs. Control; Shh vs. Control; Shh + TMZ vs. TMZ). GANT61-treated cells showed decreased MGMT, Notch1, and Hes1 mRNA expression, especially in GANT61 + TMZ (GANT61 vs. Control; GANT61 + TMZ vs. TMZ). *P < 0.05, **P < 0.01, ***P < 0.001. b Western blot analysis revealed increased MGMT, Notch1, and Hes1 protein expression in U87 and U251 glioma cells treated with TMZ. However, the GANT61 + TMZ group had lower levels of MGMT, Notch1, and Hes1 protein than the TMZ group when the same dose of TMZ was used, while GANT61 showed a lower expression than the control group and Shh + TMZ group had higher expression than the TMZ group. The results shown were representative of three independent experiments