Fig. 4

TY-011 causes accumulation of cells with ≥4 N DNA content. a MGC-803 cells were treated with indicated concentration of TY-011 for 24 h (upper panel) or with 0.2 μM TY-011 for various times (lower panel). Cell cycle distribution was assessed by flow cytometry after cells were stained with propidium iodide. Representative data were shown. b Cell cycle proportion in (a) was quantitated. Data presented are the mean ± SD of three independent experiments. c SGC-7901 cells were treated with indicated concentration of TY-011 for 24 h (upper panel) or with 0.5 μM TY-011 for various times (lower panel). Cell cycle distribution was assessed by flow cytometry after cells were stained with propidium iodide. Representative data were shown. d Cell cycle proportion in (c) was quantitated. Data presented are the mean ± SD of three independent experiments. e MGC-803 cells were treated with 0.4 μM TY-011 for 24 h, and stained with anti-α-tubulin and DAPI for microtubules and chromosome, respectively. Scale bar, 20 μm