Fig. 6

miR-19b-3p regulated TNFAIP3 expression by targeting the 3′-UTR of TNFAIP3. a Four candidate target genes of miR-19b-3p were predicted through a miRNA target prediction website. Align score, energy, and context score are all indicated. b A negative correlation was found between TNFAIP3 and miR-19b-3p in patient tissues. c Sequence alignment of the miR-19b-3p nucleotide sites with the 3′UTR of TNFAIP3 mRNA of different species. d TNFAIP3 expression and miR-19b-3p overexpression in transfected cells decreased TNFAIP3 protein expression. e The 293 T cells were transfected with miR-19b-3p or the miR-control vector and were co-transfected with the control luciferase reporter plasmid pmirGLO or with this reporter plasmid containing the TNFAIP3 wt 3′-UTR or the TNFAIP3 mut 3′-UTR. Luciferase activity was assayed 48 h later. f Biological functions of TNFAIP3 were subjected to KEGG pathway analyses