Fig. 2

Subcellular localization of CCDC88A in PDAC cells. a. Confocal immunofluorescence microscopic images of S2-013 and PANC-1 cells that were cultured with or without fibronectin and were then labeled with anti-CCDC88A antibody (green) and phalloidin (red; actin filaments). Arrows, CCDC88A localized in cell protrusions. Blue, nuclear DAPI staining. Bars, 10 μm. b. Confocal Z stack images show nuclear DAPI staining (blue), abundant cytoplasmic CCDC88A and the accumulation of CCDC88A (green) in membrane protrusions of fibronectin-stimulated S2-013 cells. Arrows, CCDC88A localized in cell protrusions. The lower and right panels in the confocal Z stack show a vertical cross-section (yellow lines) through the cells. Bar, 10 μm