Fig. 3

SW IV-134 augments gemcitabine-induce apoptotic cell death. a PANC-1 cells were treated with DMSO (control), SW IV -134 (1 μM), gemcitabine (0.5 μM), and a combination of the two drugs at their respective concentrations. Apoptotic cell death was assessed by Western blot analysis via monitoring the activation status of caspase-3 and PARP 48 hours after treatment. Beta-Actin was used a loading control. b A complementary flow cytometry-based assay was employed to assess apoptotic cell death via Annexin-V/PI staining. AsPC-1 cells were treated essentially as in (a) but SW IV-134 was (0.5 μM). After two days, the cells were submitted to Annexin-V/PI staining. Shown are representative FACS dot plots for the indicated treatment conditions (n = 4). DMSO-treated cells served as a control. The Annexin-V+/PI- cells in the lower right quadrant represent early apoptotic cells