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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Checkpoint kinase inhibitor AZD7762 strongly sensitises urothelial carcinoma cells to gemcitabine

Fig. 1

Viability and clonogenicity of UCCs and non-malignant urothelial cells after treatment with gemcitabine and/or AZD7762. a Relative cell viability in UCCs (VM-CUB1, RT-112, T24, UM-UC-3), non-malignant human urothelial cells (hTERT-NHU, HBLAK, UP) and T24rGEMCI20 cells was measured by MTT assay (mean ± SD, n = 4) after 48 h treatment with gemcitabine and/or AZD7762. b Giemsa staining of colonies from UCCs after 24 and 48 h treatments compared to DMSO solvent control. GEM stands for gemcitabine. The concentration of AZD7762 is 10 nM in RT-112 and T24 cells, and 20 nM in VM-CUB1 and UM-UC-3 cells. c Photomicrographs showing characteristic morphological changes in T24 cells treated with gemcitabine and AZD7762 (24 and 48 h) (c). Scale bar = 100 μm

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