Fig. 4
From: Checkpoint kinase inhibitor AZD7762 strongly sensitises urothelial carcinoma cells to gemcitabine
Analysis of cell death mechanism after treatments. a Relative cell viability in UCCs was measured after treatments with 10 nM gemcitabine and/or AZD7762 using the CellTiterGlo ATP-assay. The concentration of AZD7762 used was 10 nM in RT-112 and T24 cells, and 20 nM in VM-CUB1 and UM-UC-3 cells. b Induction of apoptosis was measured by caspase 3/7 assay. c Cleaved PARP was measured after the indicated treatments (24 and 48 h)