Fig. 5
From: Checkpoint kinase inhibitor AZD7762 strongly sensitises urothelial carcinoma cells to gemcitabine
Immunofluorescent analysis of gemcitabine-induced γH2A.X and 53-BP1 focus formation. a, b Immunofluorescence staining of γH2A.X (green), 53-BP1 (red), and nuclei staining with DAPI (blue) in T24 (a) and T24rGEMCI20 (b) cells after the indicated treatments. Scale bar = 50 μm. c Western blot analysis of S345 CHK1 after treatments with gemcitabine in T24rGEMCI20 cells (24 and 48 h). As loading control, GAPDH was stained