Fig. 2

CAPZA1 expression controls metastasis of HCC cells. a Western blots to detect CAPZA1 expression in HepG2 cells 24 h after siRNA transfection and in MHCC97H cells 72 h after lentiviral-mediated overexpression. b, c A CCK-8 assay was used to detect the proliferation of HepG2 cells after siRNA-mediated knockdown of CAPZA1 (b) and MHCC97H cells following CAPZA1 overexpression (c). d–f Transwell migration and invasion assays were used to detect the migration and invasion potential of HepG2cells. After 36 h for invasion assays, HepG2 invasion was enhanced by CAPZA1 siRNA (d, e), and after 18 h for migration assays, HepG2 migration was enhanced by CAPZA1 siRNA (f). g–i Transwell migration and invasion assays were used to detect the migration and invasion potential of MHCCP7H cells. After 36 h for invasion assays, MHCC97H invasion was reduced by CAPZA1-overexpression (g, j), and after 18 h for migration assays, MHCC97H migration was reduced by CAPZA1-overexpression (i)