Fig. 8
From: MIF/CD74 axis is a target for novel therapies in colon carcinomatosis
CD74 and MIF IHC staining in three additional CRC-pc samples. In the upper part of the Figure, the normal colon mucosa from the three patients is shown. In all the cases, CD74 is highly expressed in epithelial cells and in the surrounding normal cellular microenvironment. Conversely, MIF is poorly expressed. MIF staining is mainly restricted to epithelial cells. In the lower part of the figure, the corresponding peritoneal carcinomatoses (C3, C4 and C5) are shown. Immunohistochemical staining for CD74 is heterogeneous with a granular, cytoplasmic and membranous pattern in C3, while focal, granular and restricted to the inner luminal aspect in C4 (inset) and C5. MIF immunostaining is homogeneous and very low expressed in C3 compared to C4 and C5. MIF staining is mainly cytoplasmic. Images were taken using a Nikon Eclipse 80i microscope (Nikon, Tokyo, Japan) and Nikon digital sight DS-Fi1 camera equipped with control unit-DS-L2 (Nikon). Images were assembled using Adobe Photoshop 6 (Adobe Systems, San Jose, CA, USA). Original magnification at 20X