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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: FGF19/FGFR4 signaling contributes to the resistance of hepatocellular carcinoma to sorafenib

Fig. 2

FGF19 overexpression protects HCC cells against sorafenib. a–e The effect of FGF19 overexpression on Sora-induced HCC cell apoptosis and ROS generation. MHCC97L cells expressing pcDNA3.1-FGF19 (FGF19 O/E) or empty vector (EV) were treated with 4 μM of Sora over a series of time points before analysis. Cell viability was determined by MTS assays (a); cell apoptosis was determined by DAPI staining (b) and Western blot of cleaved PARP (c-PARP) (c); ROS generation was determined by DCFH-DA staining (d); and O2 •− generation was determined by electrochemical biosensor (e). In (c), expression levels were normalized against actin and reported relative to controls (fold changes shown below each lane). * p < 0.05; ** p < 0.01

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