Fig. 5

FGF19 Knockdown of in sorafenib-resistant HCC cells increases the sensitivity to sorafenib. (a) The knockdown effect of FGF19 in Sora-resistant MHCC97H (MHCC97H Sora-R) cells. (b-e) The effect of FGF19 knockdown on Sora-induced apoptosis in MHCC97H Sora-R cells. FGF19 was knocked down in MHCC97H Sora-R cells by lentiviral shRNA. FGF19 knockdown cells (shFGF19) and the control cells (shNC) were treated with different doses of Sora for 24 hours, and cell viability was determined by MTS assays (b). FGF19 knockdown cells (shFGF19) and the control cells (shNC) were treated with 20 μM of Sora over a series of time points before analysis. Apoptosis was determined by DAPI staining (c); ROS generation was determined by DCFH-DA staining (d); and O₂ ^•− generation was determined by electrochemical biosensor (e). In a, expression levels were normalized against actin and reported relative to controls (fold changes shown below each lane). * p < 0.05; ** p < 0.01