Fig. 6

Ponatinib facilitates sorafenib-resistant HCC cells to death. a-e The effect of ponatinib (Pona) on Sora-induced ROS generation and apoptosis in Sora-resistant HCC cells. Sora-resistant MHCC97H (MHCC97H Sora-R) cells were treated with 20 µM of Sora and 10 µM of Pona singly or in combination. Cell viability was determined by MTS assays (a); apoptosis was determined by DAPI staining (b) and Western blot (c); ROS generation was determined by DCFH-DA staining (d); and O2•− generation was determined by electrochemical biosensor (e). In c, expression levels were normalized against actin and reported relative to controls (fold changes shown below each lane). * p<0.05; ** p<0.01