Skip to main content
Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Oleuropein enhances radiation sensitivity of nasopharyngeal carcinoma by downregulating PDRG1 through HIF1α-repressed microRNA-519d

Fig. 3

miR-519d directly targets the 3′-UTR of PDRG1 mRNA. a Predicted miR-519d complementing sites on the 3′-UTR of PDRG1 mRNA. b Wild type (PDRG1-wt) or mutated (PDRG1-mut) 3′-UTR sequences from PDRG1 mRNA were cloned at the downstream of a luciferase reporter gene open reading frame (Luc ORF). c and d The above luciferase constructs were then transfected into NPC cells HNE-1 (c) and HONE-1 (d) expressing either negative control miR (miR-NC) or miR-519d, respectively. 24 h after transfection, luciferase activities of PDRG1-wt or PDRG1-mut constructs were measured. e and f PDRG1 mRNA (e) and protein (f) levels were analyzed in cells treated as in (c). Values were expressed as mean ± SD. ** p < 0.01, compared to miR-NC

Back to article page