Fig. 2

PFK15 suppresses the glycolytic activity of HNSCC cells. a Molecular structure of 1-(4-pyridinyl)-3-(2-quinolinyl)-2-propen-1-one (PFK15). b Cell growth determined by MTT assays in Cal27 cells after PFK15 treatment. c Measurement of fructose 2,6-bisphosphate (F26BP) in Cal27 cells after PFK15 treatment. d Glucose uptake was analyzed in Cal27 cells using 2-NBDG. e Lactate productionof Cal27 cells treated with PFK15 were detected. f ATP generation inside of the Cal27 cells treated with PFK15 were measured. Mean ± S.E.M.; *P < 0.05, **P < 0.01, ***P < 0.001