Fig. 5

PFK15 impairs the formation and function of invadopodia in Cal27 cells. a The extracellular matrix (ECM) degradation ability of Cal27 cells after PFK15 treatment were measured by seeding tumor cells on the Alexa568-gelatin coated coverslips. b PFK15 impairs the formation of invadopodia inCal27 cells, which were detected by co-localisation of cortactin, F-actin and the black holes on the Alexa568-gelatin coated coverslips. c The quantitative data of the degraded matrix on the Alexa568-gelatin coated coverslips. d The quantitative data of the invadopodia formation per Cal27 cells. e The localisation of PFKFB3 in the invadopodia of Cal27 cells. Mean ± S.E.M.; ***P < 0.001