Fig. 6

CIP4 modulates EGFR signaling and promotes MMP-2 expression in NPC cells. a and b Serum-starved 5-8F and S18 Control and KD1 cells were treated with EGF (100 ng/ml) for 0–20 min. Lysates were subjected to IB with the antibodies indicated on the right. c and d Serum-starved 5-8F and S18 Control and KD1 cells were treated with EGF (100 ng/ml) for 0–120 min. Lysates were subjected to IB with the indicated antibodies. e Densitometry was performed, and relative phospho-Erk levels are shown in the graph below (mean ± S.D.). *, P < 0.05. f IB analysis of MMP-2 and MMP-9 in 5-8F and S18 Control and KD1 cell lysates. GAPDH was used as a loading control