Fig. 3

SNHG12 regulated the expression of miR-199a/b-5p target gene and affected the NF-κB pathway. a The expressions of related snoRNAs were determined by qPCR when SK-Hep1 cells were transfected with SNHG12-siRNA. b Representative images of SNHG12 expression in human HCC tissues and adjacent non-tumor tissues by ISH assays. c Predict binding sites between SNHG12 and miR-199a/b-5p. d and e The expression of miR-199a-5p was lower in HCC tissues compared with the paired adjacent normal tissues by qPCR. f Bivariate correlation analysis of the negative association between SNHG12 expression level and miR-199a-5p expression level in 48 pairs of HCC tissues. g The miR-199a/b-5p expression level was determined by qPCR when SK-Hep1 cells were transfected with SNHG12-siRNA. h and i Western blot analysis of key molecules in NF-κB pathway after SNHG12 knockdown or miR-199a-5p overexpression in SK-Hep1 cells. Relative protein expression was identified and normalized to GAPDH. Data represented the mean ± SD from three independent experiments. *P < 0.05; NS: no significance