Effect of DOX and RES on nude mice burdened with SGC7901/DOX xenograpfts.
a Nude mice inoculated subcutaneously with SGC7901/DOX cells were treated with DOX, RES or both for 4 weeks. At the end of the treatment period, mice were sacrificed and tumor specimens harvested. The tumor volume (V) was determined by measuring the length (a) and width (b) with calipers every week and calculated using the formula: V (mm3) =1/2ab2. Mean tumor volume for each treatment group is indicated. Results are represented as the mean ± SD of 5 mice per group (n = 5,***p < 0.001). b Tumor specimens were subjected to immunohistochemical staining for PTEN,Ki67, caspase3 and vimentin. Images were captured at 400× magnification. The Scale bar represents 50 μm. Mean density of each group (Integrated optical density/Area) was presented (n = 5, *** p < 0.001; * p < 0.05;NS means not significant, p > 0.05). c A hypothetical model illustrating that RES reverses DOX-induced EMT and DOX-resistance by modulating PTEN/Akt signaling pathway. This picture was designed by J.X. and Q.Z