Fig. 2

Validation of TRIM24 as a direct target of miR-511. a Wild type (Wt) and Mutant type (Mut) TRIM24 3′UTR sequences were cloned into a psi-CHECK reporter vector. b The miR-511 expression was detected by qRT-PCR at 48 h post-transfection, U6 was used as an internal control. c The relative luciferase activity was detected by dual-luciferase reporter assay in indicated cell lines. d qRT-PCR analysis of TRIM24 mRNA expression in indicated cell lines 24 h post-transfection. e TRIM24 protein expression was detected by Western blot in indicated cell lines post-transfection. (*p < 0.05, **p < 0.01; n = 3)