Fig. 1

FoxM1 and Gli1 are highly expressed in CRC tissues. a The FoxM1 and Gli1 protein expression levels were analyzed in eight pairs of randomly selected human CRC tissues and matched adjacent non-tumor tissues by Western blotting. b Quantification of the Western blot (a). Significance was assessed using a paired samples t-test. c Immunohistochemistry staining of FoxM1 and Gli1 protein expression in a representative human CRC sample and the matched adjacent normal tissue sample in the same section. The areas of carcinoma and adjacent tissues are marked. The subcellular locations of Gli1 and FoxM1 are indicated by red arrows. d, e and f The mRNA expression level of FoxM1 analyzed in three published human CRC sample expression profiling studies using the R2 data sheet (http://r2.amc.nl). p < 0.05 was considered significant