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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Aberrant activation of hedgehog signaling promotes cell proliferation via the transcriptional activation of forkhead Box M1 in colorectal cancer cells

Fig. 4

Gli1 regulates CRC cell proliferation depending on FoxM1. a-d) LoVo cells were separately or simultaneously treated with 1 μM purmorphamine and 1 μM thiostrepton for the indicated time. a The Gli1, FoxM1, and CCNB1 protein expression levels were examined by immunoblotting after drug treatment for 48 h. b Cell viability was detected after 6 days using an MTT assay. c LoVo cells treated with indicated drugs were cultured for 2 weeks, and outgrowth colonies were stained with crystal violet. d The matched colony count of (c). Error bars represent the mean and S.D. of three independent experiments. **, p < 0.01. e and f The cell cycle profile of LoVo cells was examined by fluorescence-activated cell sorting (FACS) with propidium iodine (PI) staining after 48 h of drug treatment (e), and the percentages of multinucleate cells were quantified and are shown as a histogram (f). Purm: purmorphamine; Thios: thiostrepton. Error bars represent the mean and S.D. of three independent experiments. *, p < 0.05

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