Fig. 4

The fluorescence characterizations of nanoparticles. Imaging of MSNs was performed by CLSM (100 × objective) the loaded DOX imaging of the RDMSNs (red fluorescence), DyLight 488-avidin imaging of the RDMSNs (green fluorescence), merge imaging of RDMSNs a. The fluorescence absorbance of the RDMSNs, DMSNs and free DyLight 488-avidin were measured to analyze the rituximab conjugation efficiency. The fluorescence intensity of the RDMSN suspensions was 66.25% for the DyLight 488-labeled avidin samples, demonstrating the desired conjugation efficiency of biotinylated rituximab to the avidin-conjugated RDMSNs b