Effect of CMs from hATN and hATT in localization/expression of versican in MCF-10A, HBL100, MCF-7 and IBH-7 cells.
a-d MCF-10A, HBL100, MCF-7 and IBH-7 cells were seeded on Lab Tek Chamber Slide system and allowed to grow for 24 h. They were then incubated for 24 h with the different CMs and versican immunodetection was performed. Fluorescence was observed with confocal microscopy. Representative images are shown (scale bar: 20 μm). a MCF-10A, b HBL100, c MCF-7 and d IBH-7. Histograms show mean ± SEM of total cell fluorescence. Green: versican; red: nuclei (a.u.: arbitrary units) (n = 3, duplicate experiments) *p < 0.001 hATT-CMs and/or hATN-CMs vs. control-CMs; **p < 0.01. hATT-CMs vs hATN-CMs. e MCF-7 and IBH-7 were grown on 6 well plates, incubated for 24 h with the different CMs and cells were lysed. Versican expression was measured by Western blot. 1- cells incubated with control-CMs; 2 and 3- cells incubated with hATN-CMs; 4 and 5- cells incubated with hATT-CMs. β-actin was used as internal control. Images were analyzed by densitometry. Bars show mean ± SEM of three independent experiments. *p < 0.05 tumor cells incubated with hATT-CMs vs. hATN-CMs or control-CMs