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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Cancer cells increase endothelial cell tube formation and survival by activating the PI3K/Akt signalling pathway

Fig. 1

Phenotype changes of HUVECs after co-culture with CL1-5 cells. a Cell morphology of HUVECs co-cultured with or without CL1-5 cells, as determined by Giemsa Staining. Scale: 50 μm. b Migration ability and (d) apoptotic percentage of HUVECs after co-culture with CL1-5 cells, as measured by transwell and TUNEL assays, respectively. Green: TUNEL-positive nuclei. Red: all nuclei. *P < 0.05 compared with HUVECs only. c Phase contrast micrographs of the capillary-like tubular structures of HUVECs on Matrigel when cultured with or without CL1-5 cells for 1, 2, 6, 12, and 24 h. Bar graphs revealed the tube (upper panel) and node (lower panel) numbers (*P < 0.05). The data are presented as the mean ± SD. Experiments were performed in triplicate. Magnification, x100. H: HUVECs alone; H/C: HUVECs co-cultured with CL1-5 cells

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