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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Cancer cells increase endothelial cell tube formation and survival by activating the PI3K/Akt signalling pathway

Fig. 3

Protein changes and the F-actin distribution of HUVECs after co-culture with CL1-5 cells. a-c Immunoblots of the indicated proteins in HUVECs after interactions with CL1-5 cells for 24 h. The active Rac-1 was determined by the Active Rac1 Pull-Down and Detection Kit. β-actin or α-tubulin was used as the loading control. d Fluorescence images of lamellipodia and filopodia of HUVECs by F-actin staining. Scale: 20 μm and 10 μm (zoom in part). Red: F-actin. Blue: nuclear staining with DAPI. e The quantified number of filopodia of HUVECs co-cultured with CL1-5 cells (*P < 0.05) (n = 5). H: HUVECs alone; H/C: HUVECs co-cultured with CL1-5 cells. The data are presented as the mean ± SD. Experiments were performed in triplicate

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