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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Regulation of submaxillary gland androgen-regulated protein 3A via estrogen receptor 2 in radioresistant head and neck squamous cell carcinoma cells

Fig. 3

Impact of TAM or Fulvestrant treatment on fractionated IR of FaDu cells. a Representative pictures of an immunofluorescence staining of control (DMSO) and 10 nM Fulvestrant-treated FaDu cells with or without fractionated IR (4× 2Gy) demonstrate reduced basal and impaired induction of ESR2 protein levels (green signal) by Fulvestrant, which is confirmed by Western blot analysis (b) Cell nuclei were counterstained with Hoechst H33342 (blue signal). c Western blot analysis indicates accelerated apoptosis by the combination of fractionated IR with 1 μM TAM or 10 nM Fulvestrant, respectively, as determined by increased levels of cleaved caspase three and PARP (indicated by the arrowhead). Detection of β-Actin served as a control for protein quality and quantity. d-e Graphs represent the relative survival fraction of FaDu and Cal27 cells in a colony-forming assay after fractionated IR (4× 2Gy) and either 30 nM Fulvestrant or 1 μM TAM administration, respectively. Control-treated cells are set to one and bars represent mean values ± SEM of at least three independent experiments. * p ≤ 0.05

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