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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Che-1 sustains hypoxic response of colorectal cancer cells by affecting Hif-1α stabilization

Fig. 2

Che-1 is involved in the metabolic switch in response to hypoxia. a HCT116 cells were transiently transfected with stealth siRNA negative control (siControl) or siRNA Che-1 (siChe-1) and exposed to hypoxia for 16 h where indicated and pH was measured. b Score plots indicating metabolic differences between hypoxic and normoxic samples from HCT116 cells transiently transfected as in a and exposed to hypoxia for the times indicated. c Glucose, lactate and glutamine levels in HCT116 cells transiently transfected as in a and exposed to hypoxia for the indicated times. The levels are the means ± S.D. of four independent experiments p < 0.05). d-e HCT116 cells were treated as in A and Oxygen consumption (d) and HK activity (e) were measured. Values are mean of three independent experiments. *p < 0.05 (d) *p < 0.05, n.s., not significant (e)

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