Fig. 3

Che-1 regulates genes transcription in response to hypoxia. a Intensity Heatmap of modulated genes in HCT116 cells after 4 h of exposure to hypoxia highlighted by red frame. b Gene Ontology from Enrichr [42]. Calculated from the most significant (corrected P <0.05) 38 genes regulated in Cuff diff analysis, Normoxic cells VS Hypoxic cells. c Expression levels in HCT116 cells transiently transfected with Stealth siRNA negative control (siControl) or siRNA Che-1 (siChe-1) and exposed to hypoxia for 4 h. Gene expression levels are reported as FPKM reconstructed from Cufflinks RNA-Seq analysis. d Genome Browser screenshot of Big Wig Enrichment from RNA-Seq BAM files, showing expression of SLC2A1 (right) and SLC2A3 (left) genes in HCT116 cells transiently transfected with Stealth siRNA negative control (siControl) or siRNA Che-1 (siChe-1) and exposed to hypoxia for 4 h. e Quantitative RT–PCR (qRT–PCR) for metabolic genes expression was performed in HCT116 cells transiently transfected and treated as in c. Values were normalized to RPL19 mRNA expression. Error bars represent the standard error of three different experiments. *P ≤ 0,006, **P ≤ 0.008, ***P ≤ 0.0008. f ChIP-qPCR analysis of HCT116 cells transfected with siControl or siChe-1 exposed to hypoxia, using anti-HIF-1α Ab or control IgGs. Error bars represent the standard error of three different experiments. *P ≤ 0.004, **P = 0.0005