Fig. 6

Buformin targets mammary stem cell populations in premalignant tissues from MMTV-erbB-2 mice. Primary MECs were isolated from 18-week-old mice that were fed control or buformin diets for 10 weeks (N = 4). Cells were labeled with fluorescent antibodies for flow cytometry analysis of different MEC populations. a The CD24/CD49f-probed cells produced 3 distinct cell populations, including luminal cells (left panel), myoepithelial cells (middle panel), and MRUs (right panel) that were compared between the control-fed and buformin-fed mice. Representative images of CD24/CD49f flow cytometry plots are shown in b. All values are depicted in the appropriate graphs as the mean ± S.E. (*p < 0.05, **p < 0.01)