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Fig. 8 | Journal of Experimental & Clinical Cancer Research

Fig. 8

From: Buformin inhibits the stemness of erbB-2-overexpressing breast cancer cells and premalignant mammary tissues of MMTV-erbB-2 transgenic mice

Fig. 8

Buformin impairs the stemness of primary MECs from MMTV-erbB-2 mice. a Primary MECs were plated for a CFC assay (N = 3). After 7 days, the cells were fixed and stained as previously described. The number of colonies in the control and buformin diet samples was recorded. b Primary MECs were used for a primary mammosphere assay where the isolated cells were plated in ultra-low attachment plates for 7 days as described previously (N = 3). Then the primary spheres were counted. All values are depicted in the appropriate graphs as the mean ± S.E. (**p < 0.01)

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