Fig. 8From: Buformin inhibits the stemness of erbB-2-overexpressing breast cancer cells and premalignant mammary tissues of MMTV-erbB-2 transgenic miceBuformin impairs the stemness of primary MECs from MMTV-erbB-2 mice. a Primary MECs were plated for a CFC assay (N = 3). After 7 days, the cells were fixed and stained as previously described. The number of colonies in the control and buformin diet samples was recorded. b Primary MECs were used for a primary mammosphere assay where the isolated cells were plated in ultra-low attachment plates for 7 days as described previously (N = 3). Then the primary spheres were counted. All values are depicted in the appropriate graphs as the mean ± S.E. (**p < 0.01)Back to article page