Fig. 6
From: MicroRNA-381 inhibits the metastasis of gastric cancer by targeting TMEM16A expression
Mir-381 suppresses TGF-β signaling pathway and down-regulated EMT phenotypes. Gastric cancer cells tranfected with anomiR-381 and negative control, together with blank vector or TMEM16A overexpression plasmid. a ELISA assay indicated that supernatant concentrations of TGF-β1 and TGF-β2 dramatically reduced in agomiR-381 group, and overexpression of TMEM16A could partially rescue their expression. **P < 0.01. b QRT-PCR assay showed that mRNA of TGF-βs decreased in agomiR-381 group, which not be affected by overexpression of TMEM16A. c Protein expression of TGF-β1 and TGF-β2 in gastric cancer cells down-regulated in miR-381 overexpression group, and further decreased in TMEM16A overexpression group. d, e Transwell assay showed that the effect of miR-381 on gastric cancer cells migration and invasion could be partially recovered by supplement of TGF-β. **P < 0.01. f Western blot assay was employed to detect the expression of EMT related markers in gastric cancer cells