Fig. 6

Direct regulation of transcription factor E2F-1 on PEG10 expression. a The interference efficiency of three Si-RNAs and plasmid vector for E2F-1 was confirmed through both RT-PCR and western blotting. b, c E2F-1 could bind to the promoter of PEG10, and the binding efficiency and PEG10 protein expression was decreased or increased in E2F-1 knockdown or overexpression groups. d, e The proliferation, migration, and invasion of PC cells was affected after E2F-1 knockdown or overexpression. ^* represents P < 0.05,^** represents P < 0.01, and ^*** represents P < 0.001