Skip to main content
Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Cinchonine induces apoptosis of HeLa and A549 cells through targeting TRAF6

Fig. 4

Cinchonine inhibited AKT activation. a Cells were exposed to cinchonine (180 μM) for 0, 3, 6, 12, and 24 h. Immunoprecipitation was performed to determine the expression level of ubiquitination of AKT. Result shows that the ubiquitination-AKT was lower with the treatment of cinchonine. b Cells were pre-treated with cinchonine for the indicated durations and Western blotting was carried out to show us that cinchonine down-regulated the phosphorylation of AKT significantly at 3 and 6 h. The results shown are the mean ± SD of three independent experiments. *P < 0.05 and **P < 0.01

Back to article page