Fig. 2

Knockdown of E2F8 inhibits PTC cells proliferation and induces G1-phase arrest in vitro. a Two different effective siRNAs were used to knockdown E2F8, and the transfection efficiency was measured by western blot in both BCPAP and TPC-1. b and c Knockdown of E2F8 inhibited both BCPAP and TPC-1 cells proliferation. d and e Colony numbers of BCPAP and TPC-1 cells transfected with two siRNAs targeting E2F8 were significantly less than those transfected with siRNA-NC. f and g BCPAP and TPC-1 cells transfected with siRNA-E2F8 exhibited more arrest at G1 phase than those transfected with siRNA-NC. *p < 0.05, **p < 0.01, ***p < 0.001