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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: E2F8, a direct target of miR-144, promotes papillary thyroid cancer progression via regulating cell cycle

Fig. 4

E2F8 promotes cell cycle progression via influencing CCND1 mostly. a GO enrichment analysis showed that genes co-expressed with E2F8 are enriched in the “cell cycle” pathway. b qRT-PCR analysis of the effect of E2F8-KD on mRNA levels of CCND1, CCNE1, CCNE2, CDK1, CDK4, CDK6, p21 and p27 relative to negative control. c Western blot showed that CCND1 protein expression was downregulated in shRNA-E2F8 transfected TPC-1 cells compared with negative control. d Enforced overexpression of CCND1 (oe-CCND1) could partially reverse the shRNA-E2F8-mediated proliferation inhibition of TPC-1 cells. e Transfection efficiency of CCND1 was determined by western blot. f and g Flow-cytometry analysis showed that enforced oe-CCND1 significantly alleviated shRNA-E2F8-mediated G1-phase arrest in TPC-1 cells. *p < 0.05, **p < 0.01, ***p < 0.001, # No significance

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